Figure 3. LncRNA ANRIL could up-regulate miR-21 expression in HLECs. HLEC SRA01/04 cells were transfected with pcDNA3.1 or pc-ANRIL, and untransfected cells were acted as control. (A) and (E–G) expression of lncRNA ANRIL and miR-21 were determined by RT-qPCR. (B) Cell viability was measured by CCK-8 assay. Data are shown as the mean ± SD of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.