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Research Paper Volume 12, Issue 3 pp 3025—3041
Obg-like ATPase 1 (OLA1) overexpression predicts poor prognosis and promotes tumor progression by regulating P21/CDK2 in hepatocellular carcinoma
Figure 6. OLA1 inhibits the interaction between CDK2 and P21. (A) LM3 and MHCC-97H cells were transfected with OLA1 sh-RNA2 or treated with the P21 inhibitor UC2288 for 48 h, and cell lysates were analyzed by western blot using the indicated antibodies. (B) HCC cells were transfected with Flag-CDK2 and HA-P21 in the presence or absence of siRNA-OLA1 or GST-OLA1 for 48 h. Flag-CDK2 and HA-P21 were examined by co-IP assays, followed by western blot. (C) HEK293T cells were transfected with Flag-OLA1 or HA-P21 (or both) for 48 h. The interaction between exogenous OLA1 and P21 was examined by co-IP assays, followed by western blot. (D) The interaction between endogenous OLA1 and P21 was examined by co-IP assays, followed by western blot. h, hour.