Research Paper Volume 12, Issue 3 pp 2992—3009

Figure 7. EGF derived from hPMSCs suppressed ROS by upregulating the NRF2/HO-1 pathway in a POI mouse model. (A) qPCR analysis of the mRNA expression levels of PI3K, AKT and PTEN in POI hGCs after in vivo treatment with hPMSC or EGF (with 0.1 μg/ml, 0.5 μg/ml, and 1.0 μg/ml). (B) Western blot analysis of the protein expression levels of PI3K, AKT and PTEN in POI hGCs after hPMSC or EGF (with 0.1 μg/ml, 0.5 μg/ml, 1.0 μg/ml) treatment. (C) qPCR analysis of the mRNA levels of NRF2 and HO-1 in POI hGCs after hPMSC and EGF (with 0.1 μg/ml, 0.5 μg/ml, and 1.0 μg/ml) treatment. (D) Western blot analysis of the protein levels of NRF2 and HO-1 in POI hGCs after hPMSC and EGF (with 0.1 μg/ml, 0.5 μg/ml, and 1.0 μg/ml) treatment. All experiments were carried out three times. The error bars indicate the SD; *** p < 0.001 (compared with the POI group). POI = premature ovarian insufficiency. The qPCR primer sequences used are listed in Supplementary Table 1.