Research Paper Volume 12, Issue 3 pp 2373—2392

Figure 5. Rescue from UBE2M-knockdown induced cell proliferation inhibition and cell cycle arrest by β-catenin overexpression. MHCC-97L cells were co-transfected with the β-catenin overexpression plasmid or the empty expression plasmid in addition to the UBE2M vector or the empty expression vector. β-catenin overexpression enhanced cell proliferation and restored the suppressed effect caused by UBE2M knockdown, as revealed by CCK-8 assay (A) and colony formation assay (B). (C) β-catenin overexpression restored the inhibited G1/S transition caused by UBE2M knockdown determined by flow cytometry. (D) β-catenin, cyclin D1, c-Myc, and UBE2M expressions in MHCC-97L cells co-transfected with the β-catenin overexpression plasmid or the empty expression plasmid in addition to the UBE2M vector or the empty expression vector by Western blotting. Data was presented as mean ± SD (n = 3). *P < 0.05; **P < 0.01; P < 0.001 (student’s t-test).