Figure 2. The effects of TYRO3 knockdown or overexpression on gastric cancer (GC) cell proliferation both in vitro and in vivo. (A) Western blot analyses of TYRO3 expressions in GC cells transfected with the TYRO3-shRNA or TYRO3-constructed plasmid. (B) The effect of TYRO3-altered expression on GC cell growth using the CCK-8 assay. (C) The effect of TYRO3-altered expression on GC cell growth using the colony formation assay. (D) The effect of TYRO3 altered expression on GC cells subcutaneous xenograft growth in nude mice. Tumor mass and tumor volume are shown in the right panels (n = 6/group). (E) Immunohistochemical detection of TYRO3 and Ki-67 expression levels in xenograft tumor samples. Glyceraldehyde 3-phosphate dehydrogenase was used as a loading control. *P < 0.05.