Research Paper Volume 12, Issue 2 pp 1928—1951

Figure 8. YAP/TAZ nuclear translocation is connected with CXCL8-CXCR1/CXCR2 axis in U-87MG cell line. (A) Immunolocalization of YAP/TAZ in control and treated U-87MG cells. Ctr: Control, DF: DF2755A 0.1 μM. The nuclear fluorescence intensity quantification (indicated as mean gray value) is also reported (range of cells analysed: 167-181 for U-87MG cells). Data are mean ±SEM of three different biological replicates (n=3). Statistical analysis was performed by the unpaired Student's t-test (with Welch’s correction). *, p< 0.05, Ctr vs DF2755A were considered as statistically significant. Scale bar = 50 μm. In (B) representative western blotting and relative densitometry analysis for cytoplasmic (normalized on β-Actin) and nuclear (normalized on Lamin B1) YAP and TAZ. Data are mean ±SEM of three different biological replicates (n=3). Statistical analysis was performed by the unpaired Student's t-test (with Welch’s correction). *, p< 0.05, ***p< 0.001, Ctr vs DF2755A were considered statistically significant. Ctr: Control, DF: DF2755A 0.1 μM. R.U.: relative units.