Research Paper Volume 12, Issue 2 pp 1186—1200

Figure 4. FGF10 alleviated the cellular injury in response to PM. (A and B) FGF10 (10 ng/ml) was used to pretreat HBECs for one hour, after which these were exposed for 24 hours to 200 μg/cm³ of PM. Apoptotic death was confirmed via flow cytometry. (C) The HBECs treated with FGF10 and/or PM were assessed for viability by CCK-8 assay. (D–F) The expression of Bcl-2 and Bax were detected by western blot, with GAPDH as the loading control. (G) The HBECs that were treated with FGF10 and/or PM were collected, and the IL-6, IL-8 and TNF-α mRNA levels were measured via RT-qPCR. (H) The supernatants obtained from HBECs that were treated with FGF10 and/or PM were collected, and the IL-6, IL-8 and TNF-α levels were quantified via ELISA. Data were presented as the mean ± standard error of the mean (SEM) of three independent experiments. *P<0.05, **P<0.01 vs. control. ^#P<0.05, ^##P<0.01 vs. PM.