Research Paper Volume 12, Issue 2 pp 1141—1158

Figure 5. C1QTNF6 silence enhanced the PM-induced inflammatory responses. HBECs were transfected with C1QTNF6 siRNA or negative control siRNA, respectively and then treated with or without 300 μg/cm³ PM for 24 h. (A) Real-time PCR analysis of C1QTNF6 expression in HBECs transfected with C1QTNF6 siRNA or negative control siRNA prior to PM exposure. (B) Real-time PCR analysis of IL-1β, IL-6, and IL-8 expression in HBECs transfected with C1QTNF6 siRNA or negative control siRNA prior to PM exposure. (C) Western blot analysis of the AMPK signaling pathway activation in HBECs transfected with C1QTNF6 siRNA or negative control siRNA prior to PM exposure. The optical densities of protein bands were shown in (D). Values present as mean ± SEM; *, P<0.05, compared with the NC siRNA + PM group; #, P<0.05, compared with the NC siRNA group; n=3. HBECs, human bronchial epithelial cells; PM, particulate matter.