Research Paper Volume 12, Issue 1 pp 931—944
Upregulation of flavin-containing monooxygenase 3 mimics calorie restriction to retard liver aging by inducing autophagy
Figure 2. 40% CR for 6 months (14 to 20 months old) upregulates levels of FMO3 in liver and enhances mTOR-regulated autophagy. (A) Representative western blots of liver lysates from each group of mice, probed with the indicated antibodies. GADPH was used as the internal housekeeping protein control. Image analysis of (B) FMO3, (C) LC3, (D) p62, (E) p-mTOR/mTOR, and (F) p-S6K/S6K was performed using densitometry. Results are shown as the mean ± SD of eight animals per group. *p < 0.05 compared with the control group.