Research Paper Volume 12, Issue 1 pp 707—717

Figure 4. LncRNA KCNQ1OT1 siRNA induces miR-9 upregulation and LMX1A downregulation, promoting GC cell progression in vitro. AGS cells (A–G) or primary human GC cells (“GC-1/GC-2”) (H–L) were transfected with 500 nM of KCNQ1OT1 siRNA (“seq1/seq2”, two rounds, total 48h) or the scramble control siRNA (“si-C”, two rounds, total 48h), expression of KCNQ1OT1 (A and H), miR-9 (B and I) and LMX1A mRNA (C and J) were tested by qPCR assays; LMX1A and LMX1B protein expression was tested by Western blotting assay (C and K, LMX1A protein results were quantified); Cells were further cultured for the indicated time periods, cell viability (D) and proliferation (E and L) were tested by the listed assays; Cell migration and invasion were tested by “Transwell” (F) and “Matrigel Transwell” (G) assays respectively. For each assay, n=5. *P <0.05 vs. “si-C” cells. Experiments in this figure were repeated three times, and similar results were obtained. Bar=100 μm (E–G).