Research Paper Volume 12, Issue 1 pp 707—717

Figure 3. Forced overexpression of LncRNA KCNQ1OT1 induces miR-9 reduction and LMX1A upregulation in primary human GC cells. The primary human GC cells (“GC-1/GC-2”) were infected with LncRNA KCNQ1OT1-expressing lentivirus (“LV-KCNQ1OT1”) or scramble control vector lentivirus (“Vec”) for 72h, expression of KCNQ1OT1 (A), miR-9 (B) and LMX1A mRNA (C) were tested by qPCR assay; LMX1A and LMX1B protein expression in total cell lysates was tested by Western blotting assay (D, LMX1A protein results were quantified); Cells were further cultured for the indicated time periods, cell proliferation and viability were tested by EdU staining assay (E) and CCK-8 (F), respectively, with cell migration and invasion tested by “Transwell” and “Matrigel Transwell” assay (G and H); Cell apoptosis was quantified via the TUNEL staining assay (I) and Annexin V-FACS assay (J). For each assay, n=5. *P <0.05 vs. “Vec” cells. Experiments in this figure were repeated three times, and similar results were obtained. Bar=100 μm (E and G).