Research Paper Volume 12, Issue 2 pp 1087—1103

Figure 3. Expression of the p16^INK4a protein in articular cartilage of the destabilization of the medial meniscus (DMM) model in mice. (A) Immunohistochemical detection of p16^INK4a in tibial cartilage at 2, 5, and 10 weeks after DMM surgery. (B) Quantification of cells positively stained for p16^INK4a. ^***P < 0.001 between the two groups. And (C) the western blot analyses of the protein expression levels of p16^INK4a protein in primary chondrocytes which were induced with IL-1β (10 ng/mL) and co-cultured with metformin (1, 2, 4, and 5 mM) for 24 h 48 h and 72 h.