Figure 5. Aberrantly expressed Nanog in activated pulmonary fibroblasts and fibrotic lung tissues were mediated by Wnt/β-catenin. (A–C) The expression of Nanog in lung tissues derived from patients with idiopathic pulmonary fibrosis (IPF) was determined by Q-PCR (A), Western blot (B) and immunohistochemical analysis (C), ** P < 0.01 vs. Con. (D) The lung tissues of patients with IPF were double stained with α-SMA and Nanog by immunofluorescence. (E) The lung tissues derived from pulmonary fibrosis mouse models were double stained with α-SMA and Nanog via immunofluorescence. (F) The expression of Nanog in pulmonary fibroblasts isolated from fibrotic mouse lung tissues were measured by immunofluorescence. (G) Cells were treated as in