Expression of Concern
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This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 11, Issue 24 pp 12624—12640
Long noncoding RNA NNT-AS1 enhances the malignant phenotype of bladder cancer by acting as a competing endogenous RNA on microRNA-496 thereby increasing HMGB1 expression
Figure 2. Downregulation of NNT-AS1 inhibits the malignant characteristics of bladder cancer cells in vitro. (A) RT-qPCR was carried out to determine the expression of NNT-AS1 in T24 and TCC-SUP cells after either siNNT-AS1 or siNC transfection. *P < 0.05 vs. group siNC. (B, C) The proliferation and apoptosis status of NNT-AS1–depleted T24 and TCC-SUP cells were tested via the CCK-8 assay and flow cytometry. *P < 0.05 vs. group siNC. (D, E) The migration and invasion abilities of T24 and TCC-SUP cells after NNT-AS1 knockdown were evaluated using transwell cell migration and invasion assays. *P < 0.05 vs. the siNC group.