Research Paper Volume 11, Issue 24 pp 12452—12475

Figure 1. High-insensitive HepG2 cells do not belong to the sorafenib-resistant HepG2 cells. (A) Inhibitory effect of sorafenib treatment (24 h, 48 h and 72 h) on cell viability. The results represent as mean ± SD, n=6 per concentration. (B) Comparison of the IC₅₀ values between untreated parental cells and the daughter cells of HI_72h HepG2 cells. Two groups of untreated cells were used as controls for DMSO treatment (solvent control) and IC₈₀ of sorafenib treatment, respectively. The results represent as mean ± SD, n=5 per concentration. Dose response curves were analyzed via nonlinear regression followed by two-way ANOVA for comparisons between 2 curves, p> 0.05. (C) Workflow of TMT-based proteomic experiment. See Methods for experimental details.