Research Paper Volume 11, Issue 23 pp 11369—11381

Figure 6. Effect of circAMOTL1 on apoptosis-associated genes. MDA-MB-231 cells transfected with or without circAMOTL1 plasmid were treated with PAX (1 μg/ml) for 24 hours. One group of PAX-treated cells were subjected to Triciribine (1 μM) treatment. The mRNA expression of BCL2 (A), BAX (B) and BAK (C) was detected with RT-PCR. n=4. **p<0.01 compared to untreated vector control. ^##p<0.01 compared to PAX-treated vector control. In addition, MDA-MB-231 cells were also transfected with negative control or circAMOTL1 siRNA (si-circAMOTL1) followed by PAX treatment for 24 hours. The mRNA expression of BCL2 (D), BAX (E) and BAK (F) was detected with RT-PCR. n=4. *p<0.05, **p<0.01 compared to untreated negative control. ^#p<0.05, ^##p<0.01 compared to untreated si-circAMOTL1. (G) The protein expression of apoptosis-associated proteins, such as BCL2, BAX and BAK was detected in vector control and circAMOTL1-transfected cells treated with or without PAX. (H) The protein expression of apoptosis-associated proteins BCL2, BAX and BAK was detected in negative control and circAMOTL1 siRNA-transfected cells treated with or without PAX. (I–K) Quantitation of the intensities of protein bands of BCL2 (I), BAK (J) and BAX (K) in Panel G. (L–N) Quantitation of the intensities of protein bands of BCL2 (L), BAK (M) and BAX (N) in Panel H.