Research Paper Volume 11, Issue 23 pp 11136—11147

Figure 5. Ninj2 shRNA or KO induces apoptosis activation in human glioma cells. A172 glioma cells (A–E), U251MG glioma cells (F and G) or the primary human glioma cells (derived two patients, “P1/P2”, F and G) were transduced with lentiviral Ninj2 shRNAs (“sh-Ninj2”, two different sequences “Seq1/Seq2”), control shRNA (“sh-C”) or the CRISPR/Cas9 Ninj2 KO construct (“ko-Ninj2”), stable cells were established via puromycin selection; The relative caspase-3 activities were tested (A and F); Cell apoptosis was tested by TUNEL staining (C and G) and Annexin V FACS (D and E); Expression of listed proteins was tested by Western blotting (B). Expression of listed proteins was quantified and normalized to the loading control (B). For each assay, n=5. *p<0.05 vs. “sh-C” cells. Experiments in this figure were repeated five times, and similar results were obtained.