Research Paper Volume 11, Issue 23 pp 10902—10922

Figure 6. SNHG12 promotes the progression of GC by activating the PI3K/AKT pathway. (A, B) Western blot analysis was performed to detect PI3K/AKT pathway and downstream protein levels in AGS and MGC-803 cells transfected with si-SNHG12, and in BGC-823 cells transfected with pc-SNHG12. (C, D) LY294002 inhibited the expression of p-PI3K and p-AKT in BGC-823 and SNHG12-overexpressing BGC-823 cells. (E) The effects of LY294002 on the proliferation of BGC-823 and SNHG12-overexpressing BGC-823 cells were detected with a CCK-8 assay. (F) The effects of LY294002 on the invasion of BGC-823 and SNHG12-overexpressing BGC-823 cells were detected with a Transwell assay. (G) The effects of LY294002 on the apoptosis of BGC-823 and SNHG12-overexpressing BGC-823 cells were detected by flow cytometry. Data are shown as the mean ± SEM of at least six experiments. *P<0.05, **P<0.01, ***P<0.001.