Research Paper Volume 11, Issue 22 pp 10664—10683

Figure 5. (A) The binding region between Meg3 and miR-93-5p were predicted, and the sequences of wild-type Meg3 (WT-Meg3) or mutant Meg3 (MUT-Meg3) sequences were shown. (B) The directly binding between Meg3 and miR-93-5p were confirmed by a luciferase reporter assay was performed with the luciferase reporter plasmids of WT-Meg3 or MUT-Meg3. (C) RNA immunoprecipitation (RIP) was performed using input from cell lysate, IgG, or anti-Ago2. The relative expression levels of Meg3 and miR-93-5p were detected by qPCR. (D) The inhibitory efficiency of miR-93-5p inhibitor and its effects on Ereg expression were determined by qRT-PCR. (E) The influences of miR-93-5p inhibitor on the expression of inflammatory cytokines were determined by qRT-PCR. (F) The influences of miR-93-5p inhibitor on the protein expression of Ereg, MMP1 and MMP3 were determined by WB.