Research Paper Volume 11, Issue 23 pp 10826—10838

Figure 2. Silencing circ5912 promotes bladder cancer cell growth and metastasis in vitro. Two siRNAs that targeted circ5912 were designed and synthesized. (A) qPCR detected levels of circ5912 and FIP1L1 after treatment with the siRNAs; (B, C) a CCK8 assay was performed to evaluate cell viability; (D, E) a clone forming assay was performed to detect the ability of self-renewal; (F–H) wound healing ability was measured by the distance between the two sides of induced injury after 24 hours, scale bar: 100μm; (I–L) migration and invasion were assessed by counting cells that were able to penetrate the trans-well membrane, scale bar: 25μm.