Research Paper Volume 11, Issue 24 pp 11829—11843
Suppression of FADS1 induces ROS generation, cell cycle arrest, and apoptosis in melanocytes: implications for vitiligo
Figure 2. Expression of FADS1 in human vitiligo specimens and melanocyte cell lines. (A) Relative expression of FADS1, HACD2, and ELOVL5 in vitiligo samples (P) and matched normal tissues (N) by qRT-PCR (n = 9). (B) Free fatty acid in vitiligo samples (P) and matched normal tissues (N) detected by ELISA kit. (C) FADS1 expression in the vitiligo melanocyte cell line PIG3V and in the normal epidermal melanocyte cell line PIG1 detected by qRT-PCR. (D) FADS1 expression by qRT-PCR in PIG1 cells transfected with NC-siRNA, FADS1-siRNA1, or FADS1-siRNA2 for 72 h. (E) FADS1 expression by qRT-PCR in PIG3V in cells transfected with lenti-NC or lenti-FADS1 for 72 h. **P < 0.01, compared with NC cells.