IL-10 produces a dual effect on OGD-induced neuronal apoptosis of cultured cortical neurons via the NF-κB pathway

Hongbin Chen; Wei Lin; Peiqiang Lin; Mouwei Zheng; Yongxing Lai; Manli Chen; Yixian Zhang; Jianhao Chen; Xiaohui Lin; Longzai Lin; Quan Lan; Qilin Yuan; Ronghua Chen; Xinhong Jiang; Yingchun Xiao; Nan Liu

doi:doi:10.18632/aging.102411

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Research Paper Volume 11, Issue 23 pp 10796—10813

IL-10 produces a dual effect on OGD-induced neuronal apoptosis of cultured cortical neurons via the NF-κB pathway

Figure 1. OGD-induced peaks of p65 and c-Rel at different time points in cultured primary cortical neurons after OGD injury. (A) The representative image of western blot analysis for p65 and c-Rel. (B) Western blot analysis of p65 (n=3). *p<0.001, as compared with Control group; ^#p<0.001, as compared with OGD/R3h group; **p<0.001, as compared with OGD/R3h group; ^##p<0.001, as compared with OGD/R3h group; ***p<0.001, as compared with OGD/R3h group; ^###p<0.001, as compared with OGD/R3h group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F=61.131, p<0.0001. (C) Western blot analysis of c-Rel (n=3). *p>0.05, as compared with Control group; ^#p<0.001, as compared with OGD/R24h group; **p<0.001, as compared with OGD/R24h group; ^##p<0.001, as compared with OGD/R24h group; ***p<0.001, as compared with OGD/R24h group; ^###p<0.001, as compared with OGD/R24h group; by one way analysis of variance (ANOVA) followed by Student-Newman-Keuls multiple comparisons test, F=78.545, p<0.0001.