Research Paper Volume 11, Issue 21 pp 9643—9660

Figure 1. USP22 mRNA and protein levels in GC tissues and cell lines. (A) Histogram plots show USP22 mRNA levels based on qRT-PCR analysis in 40 pairs of GC (C) and adjacent noncancerous (N) tissues. GAPDH was used as an endogenous control. (B) Representative images show western blot analysis of USP22 protein levels in eight pairs of GC (C) and adjacent noncancerous tissues (N). β-actin was used as endogenous control. (C–D) The levels of USP22 mRNA (C) and protein (D) in GC cell lines and normal gastric mucosal epithelial cells are shown in the histogram plots and representative images, respectively. GAPDH and β-actin were used as the controls in the qRT-PCR and western blot analysis, respectively. Data are expressed as mean ± SD of three replicates. *p < 0.05 is considered statistically significant.