Expression of Concern
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This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 11, Issue 18 pp 7830—7846
Downregulation of LncRNA-XIST inhibited development of non-small cell lung cancer by activating miR-335/SOD2/ROS signal pathway mediated pyroptotic cell death
Figure 3. The effects of LncRNA-XIST on NSCLC cell apoptosis. (A) FCM was used to detect A549 cell apoptosis and (B) quantification was conducted. (C) The expression levels of apoptosis associated proteins (Caspase-3, Bax and Bcl-2) in A549 cells, which were normalized to β-actin and (D) quantified by Image J software. (E) FCM was used to detect H1299 cell apoptosis and (F) quantification was conducted. (G) Western Blot was used to detect Caspase-3 levels in H1299 cells, which were normalized to β-actin and (H) quantified by Image J software. (I, J) The apoptosis ratio of HBE cells was detected by FCM. (“NS” represented no statistical significance, “*” represented p < 0.05, “**” represented p < 0.01).