Research Paper Volume 11, Issue 18 pp 7746—7779

Figure 2. The modulation of THs alters glucose homeostasis in adulthood. (A) Circulating glucose levels during an OGTT. n = 8 per group. Repeated measurements two-way ANOVA. See also Supplementary Figure 2A. (B) AUC for the OGTT. Two-way ANOVA. (C) Circulating insulin levels during an OGTT. n = 8 per group. Repeated measurements two-way ANOVA. See also Supplementary Figure 2B. (D) AUC for the insulin levels in the OGTT. Two-way ANOVA. (E) Circulating glucose levels during an IPPTT. n = 8 per group. Repeated measurements two-way ANOVA. See also Supplementary Figure 2C. (F) AUC for the IPPTT. Two-way ANOVA. (G) Circulating glucose levels during an ITT. n = 8 per group. Repeated measurements two-way ANOVA. See also Supplementary Figure 2D. (H) AUC for the ITT. Two-way ANOVA. (I) Western blots showing total Akt and pSer473 Akt in liver extracts of Wt and Pax8 +/- mice. Mice were 9 month-old at time of death and were fasted 16 hours prior killing. (J) Densitometric analysis of western blots shown in panel I. n = 6 per group. T-test two tailed. (K) Western blots showing total Akt and pSer473 Akt in gastrocnemius extracts of Wt and Pax8 +/- mice. Mice were 9 month-old at time of death and were fasted 16 hours prior killing. (L) Densitometric analysis of western blots shown in panel K. n = 6 per group. T-test two tailed. (M) Glucose levels in blood after 16 hours of fasting. n = 8 per group. Two-way ANOVA. (N) Circulating insulin levels after 16 hours of fasting. n = 8 per group. Two-way ANOVA. (O) HOMA-IR. n= 8 per group. Two-way ANOVA. (P) Percentage of HbA1c. n = 7 per group. Two-way ANOVA. Data are represented as the mean ± SEM. Unless otherwise stated, mice were 7-month old at the time of experimentation. h: hours. a.u.: arbitrary units. * p-value < 0.05 between Wt mice and Pax8 +/- mice. & p-value < 0.05 between Wt mice and Wt T4 mice. # p-value < 0.05 between Wt mice and Pax8 +/- T4 mice.