Figure 7. Effect of exercise and cardiac Sir2 on the climbing index and average lifespan in Drosophila. (A) The climbing index. The sample size was about 100 flies for each group. (B) The average lifespan. The sample size was 200–220 flies for each group. (C) Percents of survival curve. The rightmost curve represents the cardiac dSir2 overexpression combined with exercise group, of which flies had the longest lifespan. P-values for lifespan curves were calculated by the log-rank test. Data are represented as means ± SEM. *P<0.05; **P <0.01.