Figure 4.Curcumin increased the expression of retrograde axonal transport molecular motor and scaffolding proteins, decreased the expression of forward axonal transport molecular motor and promoted autophagy flux in N2a/APP695swe cells. (A–F) Western blot analysis of retrograde axonal transport molecular motor DIC (A–B), DHC1, DLC-3 (C–D) and forward axonal transport molecular motor KIF (E-F) in each group; The data represent as mean ± SEM of a typical series of 3 experiments (# P<0.05, compared to the WT group; * P<0.01, compared to the APP or DMSO group). (G–H) Western blot analysis of P62 and LC3II in each group with(out) dynein inhibitor EHNA; The data represent as mean ± SEM of a typical series of 3 experiments (# P<0.05, compared to the WT group; * P<0.01, compared to the APP or DMSO group; **, P<0.01, compared to the EHNA group; ##, P<0.01, compared to the EHNA group). (I–J) Western blot analysis of scaffolding proteins Huntingtin and RILP in each group; The data represent as mean ± SEM of a typical series of 3 experiments (# P<0.01, compared to the WT group; * P<0.001, compared to the APP or DMSO group).
