Research Paper Volume 11, Issue 17 pp 7036—7050

Enhancing the retrograde axonal transport by curcumin promotes autophagic flux in N2a/APP695swe cells

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Figure 4. Curcumin increased the expression of retrograde axonal transport molecular motor and scaffolding proteins, decreased the expression of forward axonal transport molecular motor and promoted autophagy flux in N2a/APP695swe cells. (AF) Western blot analysis of retrograde axonal transport molecular motor DIC (AB), DHC1, DLC-3 (CD) and forward axonal transport molecular motor KIF (E-F) in each group; The data represent as mean ± SEM of a typical series of 3 experiments (# P<0.05, compared to the WT group; * P<0.01, compared to the APP or DMSO group). (GH) Western blot analysis of P62 and LC3II in each group with(out) dynein inhibitor EHNA; The data represent as mean ± SEM of a typical series of 3 experiments (# P<0.05, compared to the WT group; * P<0.01, compared to the APP or DMSO group; **, P<0.01, compared to the EHNA group; ##, P<0.01, compared to the EHNA group). (IJ) Western blot analysis of scaffolding proteins Huntingtin and RILP in each group; The data represent as mean ± SEM of a typical series of 3 experiments (# P<0.01, compared to the WT group; * P<0.001, compared to the APP or DMSO group).