Figure 6. NRF2 suppressed EPC senescence. (A–D) β-galactosidase staining of Y, MA, A, A+tBHQ, siNC and siNrf2 EPCs. (E) Expression of Nrf2, Ho-1, Nqo-1, Keap1, p65, Trx, Nlrp3, Txnip, p21 and p16 mRNA in Y, MA, A, A+tBHQ, siNC and siNrf2 EPCs. (F–K) Representative Western blots and quantitative analysis of NRF2, KEAP1, p65, TRX, NLRP3, TXNIP, p21 and p16 levels in Y, MA, A, A+tBHQ, siNC and siNrf2 EPCs. *P<0.05. Y, young; MA, middle-aged; A, aged. A+tBHQ, aged EPCs treated with tBHQ.