Figure 7. (A) The detection of SMC of the artery wall by immunohistochemical staining with anti-SMC monoclonal antibody. (Gross appearance, 30x, 100x). (B) The detection of SMC content of the artery wall by immunofluorescence assay. (Gross appearance, 30x). (C) Relative expression of TPM2 by RT-qPCR analysis. *p< 0.05, compared with control. (D) Western blotting expression of TPM2 in the control (Con) and atherosclerosis (AS) groups. (E) Quantitative comparison of TPM2 expression between the two groups. (F) The linear correlation between intima-media thickness and the relative expression of TPM2. (G) The linear correlation between intima-media thickness and the relative expression of SMC. (H) The linear correlation between the relative expression of TPM2 and the relative expression of SMC. SMC: smooth muscle cell.