Research Paper Volume 11, Issue 15 pp 5769—5785

Figure 1. Combination treatment of lonafarnib and sorafenib inhibits HepG2 cell growth. (A and B) HepG2 and MIHA cells were subjected to CCK-8 assay with escalatory concentrations of lonafarnib or sorafenib. The IC50 value at 48 h was determined in these cell lines: HepG2 (lonafarnib: 15.6 μM, sorafenib: 12.6 μM), MIHA (lonafarnib: 38.5 μM, sorafenib: 38.4 μM). (C) Dose escalation effect of lonafarnib and sorafenib on the viability of HepG2 and MIHA cells measured at 48 h by CCK-8 assay. ns, P > 0.05; *P < 0.05; ***P < 0.001. (D) Colony formation assay in HepG2 cells. Cells were treated with lonafarnib (10 μM) and/or sorafenib (5 μM) for 14 days. At the end of this period, cells were stained with 0.5% crystal violet. (E) The number of colonies is calculated and presented as the means ± SD of triplicates. **P < 0.01; ***P < 0.001. (F) Representative image of TUNEL assay. HepG2 cells were maintained in 10 μM lonafarnib and/or 5 μM sorafenib for 48 h. The green puncta indicate the broken DNA fragment in cells. (G) The number of TUNEL-labeled DNA fragments were presented as the means ± SD of triplicates. ***P < 0.001.