Research Paper Volume 11, Issue 17 pp 6657—6673

Figure 5. DSP inhibits GC growth and metastasis via Wnt/β-catenin signaling. (A) Depletion and overexpression of DSP were verified using RT-qPCR and WB. (B) CCK8 assays were performed after knockdown and overexpression of DSP (**P < 0.01). (C) Flow cytometric apoptosis assays were used to assess cell apoptosis after silencing and overexpression of DSP (**P < 0.01). (D) Transwell assays were used to determine the invasiveness of cells with down-regulated and up-regulated DSP (*P < 0.05, **P < 0.01). (E–F) Total, cytoplasmic and nuclear levels of β-catenin and lamin B1 were detected by WB after promoting or inhibiting DSP expression. (G) Expression levels of E-cadherin, N-cadherin, vimentin and c-myc were determined using WB after DSP knockdown or combined treatment with DSP siRNA and a Wnt/β-catenin inhibitor (XAV939a).