Research Paper Volume 11, Issue 13 pp 4706—4719

Figure 5. Effects of TP on the distribution of CGs and mitochondria in DDP-exposed porcine oocytes. (A) Representative images of CGs localization in control, DDP-exposed and TP-supplemented oocytes. Scale bar, 40 μm. (B) The fluorescence intensity of cortical granules was measured in control, DDP-exposed and TP-supplemented oocytes. (C) Representative images of mitochondria in control, DDP-exposed and TP-supplemented oocytes. Scale bar, 35 μm. (D) The immunofluorescence intensity of mitochondrion signals was recorded in control, DDP-exposed and TP-supplemented oocytes. (E) Mitochondrial membrane potential (ΔΨm) was detected by JC-1 in control, DDP-exposed and TP-supplemented oocytes (Red, high ΔΨm; Green, low ΔΨm). (F) The ratio of red and green fluorescence intensity was recorded in control, DDP-exposed and TP-supplemented oocytes. Data were presented as mean percentage (mean ± SEM) of at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.