Research Paper Volume 11, Issue 13 pp 4382—4406

Figure 6. Cell cycle negative regulators expression levels and SASP pro-inflammatory cytokines transcripts are up regulated by C5a. (A–B) Representative p21 Waf1/Cip1 and p16INK4a western blot of C5a-stimulated RTEC and quantification. Protein expression was normalized to βactin. (C–I) Time course of IL-6, MCP1, CTGF, SERPIN1 (PAI-1), BCL9, CYP1B1 and CDK6 gene expression in RTEC stimulated for 3h or 24h by C5a followed or not by 24h or 48h of normal culture was assessed by qPCR. Data were normalized to GAPDH. *p<0.05, **p<0,01, n=3. H₂O₂ (100-300 μm) was used as positive control of senescence.