Research Paper Volume 11, Issue 12 pp 4050—4065

Figure 5. TRPM7 overexpression reverses proliferation and apoptosis resistance in PASMCs. (A) PASMCs from rats were infected with adenovirus expressing control vector (Ad-Ctrl) or TRPM7 (Ad-TRPM7). After 72 h of infection, the protein level of TRPM7 was determined by Western blot analysis. β-actin was used as a loading control. The representative images from 3 independent experiments are shown. (B–D) PASMCs from rats were infected with Ad-Ctrl or Ad-TRPM7. After 24 h of infection, cells were serum starved for 24 h, followed by incubation with medium containing 5% FCS for another 24 h. (B) Cell proliferation was determined by BrdU incorporation assay. (C) Cell apoptosis was detected by TUNEL staining. Results are expressed as a percentage relative to control. Data are mean ± SD. n = 3. One-way ANOVA test. **, P < 0.01 compared to vector. (D) The protein expression of cleaved caspase-3 was determined by Western blot analysis. β-actin was used as a loading control. The representative images from 3 independent experiments are shown.