Research Paper Volume 11, Issue 11 pp 3851—3863

Figure 3. The effect of TRF on markers of liver aging in rats exposed to ALF or TRF for 18 months and continued on ALF for additional 3 months. (A, B and C) SA-β-gal staining. Percent area positive for SA-β-gal staining was decreased in TRF compared to ALF group (**p- value <0.01). (D and E) Western blot analysis of SIRT1 expression. A significant up-regulation was seen in TRF group (**p-value <0.01). (F, G and H) Immunofluorescence analysis of intracellular lipid accumulation (red) in the liver. Nuclei were stained with Hoechst (blue). A decrease in lipid droplets was evident in TRF group (*p- value <0.05).