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Research Paper Volume 11, Issue 11 pp 3668—3678
Apigenin inhibits growth and migration of fibroblasts by suppressing FAK signaling
Figure 2. Apigenin suppresses the kinase activity of FAK and inhibits FAK phosphorylation. (A, B) Western blot and real-time PCR analyses showing the Collagen I and FAK content in normal skin tissue, normal scar tissue and hypertrophic scar tissue. ** P < 0.05 vs. hypertrophic scar tissue. (C) Kinase Glo luminescence assays showing the inhibitory effect of apigenin on the kinase activity of FAK; 2 μM ALX was used as an activated control. Data are shown as the mean ± SD. ** P < 0.05 vs. NaCl-treated FAK group. (D) Kinase Glo luminescence assays comparing the inhibitory effects of apigenin on the kinase activities of FAK-WT and FAK-KM(Y397A); 2 μmol ALX used as an activated control. Data are shown as the mean ± SD. ** P < 0.05 vs. NaCl-treated FAK-WT group; ## P < 0.05 vs. NaCl-treated FAK-KM (Y397A) group. (E) Kinase Glo luminescence assays examining the inhibitory effects of apigenin on the kinase activities of FAK in HSFs transfected with si-FAK; 2 μmol ALX used as an activated control. Data are shown as the mean ± SD. ** P < 0.05 vs. NaCl-treated FAK group. (F–I) Western blot and real-time PCR analyses of FAKp-Tyr397 and FAK levels in HSFs and HKFs treated for 24 h with NaCl or the indicated concentration of apigenin. **P < 0.05 vs. NaCl. (J, K) Western blot and real-time PCR analyses of the effect of 20 μM apigenin FAKp-Tyr397 and FAK levels in HSFs overexpressing empty vector or FAK. **P < 0.05 vs. NaCl group; ## P < 0.05 vs. NaCl with FAK-overexpressed group. (L, M) Western blot and real-time PCR analyses of the effect of 20 μM apigenin on FAKp-Tyr397 and FAK levels in HSFs transfected with si-FAK or si-NC (negative control). **P < 0.05 vs. NaCl group.