Research Paper Volume 11, Issue 11 pp 3601—3623

Figure 3. RHCG is downregulated in HNSCC tissues and cell lines. (A) Western blotting analysis of RHCG protein levels in HNSCC and normal tissues (n=3). (B) mRNA expression of RHCG in HNSCC and corresponding normal tissues (n=20). (C) Expression of RHCG as examined by immunohistochemistry (×400) in in HNSCC and normal tissues. (D) Western blotting analysis of RHCG expression in a human immortalized oral epithelial cell (HIOEC) line and HNSCC cell lines. (E) Real-time PCR analysis of relative RHCG mRNA expression levels HIOEC cells and HNSCC cell lines. (F) Comparison of RHCG expression in cancerous tissues and normal tissues in 4 different cohorts, including Ginos Head-Neck, Peng Head-Neck, Toruner Head-Neck and Ye Head-Neck according to the Oncomine database. (G) Meta-analysis of RHCG expression across the 4 analyses. (H) RHCG protein expressed in normal and HNSCC cancerous tissues from The Human Protein Atlas database. The tissue expressing abundant RHCG signals was normal tongue tissue from a female at an age of 73. RHCG was strongly stained in squamous epithelial cells, mainly located in cytoplasmic or membranous region; the tissue with no staining was head and neck squamous cell carcinoma tissue from an 81-year-old woman.