Research Paper Volume 11, Issue 9 pp 2852—2873

Figure 3. Demonstration of primer MARylation in Mmu WCE (A) and in the system reconstituted from recombinant proteins (B). (A) Mmu cell extract proteins (0.5 mg/mL) were incubated for 5 min with 100 nM DNA duplexes bearing dRP, pDEG, or flap in the presence of 5 mM MgCl₂ and 0.5 mM NAD⁺ in the absence or presence of PARG and olaparib. (B) Recombinant proteins were incubated for 10 min with 100 nM DNA duplexes bearing dRP, pDEG, or flap in the presence of 5 mM MgCl_2, 0.1 mM dNTPs, and 0.5 mM NAD⁺ (when indicated). Lanes 1 in A and B correspond to the initial primer (control).