Research Paper Volume 11, Issue 9 pp 2699—2723

Figure 4. Elevated laminins (LMs) in senescent HLE B-3 cells and cell basement membranes (BMs) induced by H₂O₂. Cells (or cell BMs) of the control group were cultured in medium only, while cells (or cell BMs) of senescent group were cultured in medium with H₂O₂ (400 μM) for 96 h. (A) Total LM in HLE B-3 cells as detected by ELISA (data were analyzed by paired t-test). (B) Immunoblot analysis of 11 LM subunits, collagen 1α1, ATP1A1, MMP-9, and TGF-β1 in HLE B-3 cells. (C) Total LM in HLE B-3 cell BMs as detected by ELISA (data were analyzed by paired t-test). (D) SDS-PAGE analysis followed by CBB staining of HLE B-3 cell BMs. (E) Immunoblot analysis of LM subunits and MMP-9 in HLE B-3 cell BMs. (F) Immunofluorescence analysis of LMα4, LMβ3, and MMP-9 in HLE B-3 cell BMs (Scale bars: 50 μm). (G) TGF-β1 in HLE B-3 cell BMs, as detected by ELISA (data were analyzed using One-way ANOVA). Data are shown as mean ± SD. *, p<0.05; **, p<0.01 versus control group.