Research Paper Volume 11, Issue 9 pp 2699—2723

Figure 3. Premature senescence model of human lens epithelial cells (HLE B-3) induced by H₂O₂. Cells of the control group were cultured in medium only, whereas cells of senescent group were cultured in medium with H₂O₂ for 96 h. (A) Viabilities (left) of HLE B-3 cells treated with different concentrations of H₂O₂ (0–600 μM) for 96 h, as measured via an MTT assay. Morphologic changes (right) of HLE B-3 cells following a 96 h exposure to 400 μM H₂O₂. (B) Percentage of SA-β-gal-positive cells in HLE B-3 cells treated with different concentrations of H₂O₂ (0–600 μM) (left). SA-β-gal activity as measured by cell staining (right). (C) Immunoblot analysis of GLB1, p21 and P53 in HLE B-3 cells. (D) Immunofluorescence analysis of p21 (green) in HLE B-3 cell nuclei. (Scale bars: 100 μm). Data were shown as mean ± SD and are representative of 3 independent experiments.