Expression of Concern
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This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 11, Issue 3 pp 921—932
MicroRNA-141 suppresses growth and metastatic potential of head and neck squamous cell carcinoma
Figure 2. EGFR is a direct target of miR-141. (A) Prediction of miR-141 binding sites in the EGFR 3’-UTR. (B) Interaction between miR-141 and the EGFR 3’-UTR in FaDu and Cal-27 cells tested in luciferase assays. AS, antisense. (C) Western blots showing expression of EGFR in INOK, FaDu and Cal-27 cells. (D) Western blots showing expression of miR-141 and EGFR in INOK, FaDu and Cal-27 cells. (E, F) Western blot and real-time PCR showing co-expression of EGFR in FaDu cells with miR-141 mimic or inhibitor. (G, H) Western blot and real-time PCR showing co-expression of EGFR in Cal-27 with miR-141 mimic or inhibitor. Bars depict the mean ± SD. ** P< 0.05 vs. INOK cells (C, D) or control (E, F).