Research Paper Volume 11, Issue 1 pp 230—239

Figure 2. Longer lifespan induced by the change of Rpd3 and Loco results from the reduced catabolism. (A) The gene expression analyses (Rpd3-Down or Loco-Up/control). From RNA-seq experiments using 2-day-old male flies, the genes changed more than 1.3-fold were selected with p-value <0.05 (Fisher’s exact test). The underlined genes represent that their expressions decrease (green) and increase (purple) in a different group, respectively. (B) With the genes oppositely changed in the Rpd3-Down and Loco-Up (*: total 39 in A), the gene ontology was analyzed using a DAVID web tool (http://david.abcc.ncifcrf.gov/home.jsp). %: involved genes/total 39 genes; p-value: a modified Fisher Exact. (C) Expressional changes of selected genes in RNA-seq and real-time PCR analyses. The fold changes were averaged from 4 ~ 13 independent experiments using four different RNA batches. Bold change: p-value < 0.05 ~ 0.0001. (D) Functional analyses (http://flybase.org) of genes which were selected with more than 1.5-fold changes and p-value <0.05 in both Rpd3-Down (longer lifespan) and Loco-Up (shorter lifespan) groups. *: genes tested as a representative of each functional subgroup with RT-PCR analysis in (C).