Figure 3. Overexpression of miR-542-3p induced apoptosis and cell cycle was arrested in osteosarcoma cells. (A) After transfection, the cells were collected and stained with Annexin V-FTIC/PI. The percentages of early (low right quadrant) and late apoptotic cells (upper right quadrant) were assessed by flow cytometry. **P<0.01 compared with the NC group; (B) The effect of miR-542-3p on cell cycle progression of 143B and U-2OS cells after 48h incubation was detected by flow cytometry analysis. *P<0.05, **P<0.01 compared with the NC group.