Figure 5. Sirt1 deacetylates 9G8 at K24. (A) Mass spectra obtained from HA-9G8 immunoprecipitated from HEK-293T cells co-transfected with pcDNA3.1/SIRT1 or control DNA. (B) Sirt1 was expressed alone or co-overexpressed with 9G8K24R in HEK-293T cells transfected with pCI/SI9-SI10. After 48 h transfection, the alternative splicing products of tau exon 10 were analyzed by RT-PCR. The data are presented as mean ± S.D. (n=3) and analyzed with two-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with control group; ## p < 0.01, compared with 9G8 group.