Research Paper Volume 10, Issue 6 pp 1390—1401

Figure 5. Effect of ETS-2 on the pGL-lair1-p6 (-256/-8) and ETS binding site mutant constructs. (A) DNA sequence of the LAIR-1 gene basic promoter (-256 to -8 relatives to the translational start site); The ETS binding sites A, B and C predicted by corresponding software are shown with bold-type letter. (B) Putative ETS binding site (A, B and C) deletion mutagenesis was carried out in the construct pGL-lair1-p6 (-256/-8). Expression vector of ETS-2 (pSPORT-ETS2) was co-transfected with the pGL-lair1-p6 (-256/-8) or other different mutant constructs, into HEK293T cells. Luciferase activities were measured at 48 h after transfection. The bars present the mean ± standard deviation of three independent transfection experiments (a, b, c, d, and e indicate significant differences in groups identified with 1-way ANOVA, p<0.05).