Figure 6. Analysis of differential miRNA expression in vitamin D sufficient and insufficient mice. Total RNA was isolated from tibialis anterior muscle and analyzed using miRNA sequencing. A total of 202 unique miRNAs reached thresholds for analysis, and a heat map was generated from 15 miRNA with a p<0.05 for differential expression between vitamin D sufficient (STD) and insufficient (LOW) mice (n=6). Further analysis for false positives (Q-val) revealed mir-26a as being differentially expressed between the two groups.