Research Paper Volume 10, Issue 3 pp 463—480
Protective effects of melatonin against nicotine-induced disorder of mouse early folliculogenesis
Figure 8. Melatonin suppresses autophagy in nicotine-exposed ovaries. (A) Protein level of LC3-2/LC3-1 and Beclin-1 in control, intraperitoneally injected 1 mg /kg nicotine and intraperitoneally injected 1 mg /kg nicotine plus 1 μM melatonin pups were detected by western blot. (B) Autophagosomes (arrow) in the somatic cell and oocyte cytoplasm in control, intraperitoneally injected 1 mg /kg nicotine and intraperitoneally injected 1 mg /kg nicotine plus 1 μM melatonin pups ovaries with TEM. The results are presented as mean±SD. All the experiments were repeated at least three times. * P < 0.05; ** P < 0.01.