Research Paper Volume 10, Issue 3 pp 322—338

Figure 3A. EC-specific knockdown of DNA damage response (DDR)-related factors causes an increase in the age-related phenotypes of ISCs. EC-specific knockdown of Mre11, Rad50, Nbs1, ATM, ATR, Chk1, or Chk2 induce DNA damage accumulation in ISCs. Flies carrying Myo^ts>GFP, Myo^ts>GFP+Mre11i, Myo^ts>GFP+Rad50i, Myo^ts>GFP+Nbs1i, Myo^ts>GFP+ATMi, Myo^ts>GFP+ATRi, Myo^ts>GFP+Chk1i, or Myo^ts>GFP+Chk2i genotypes were cultured at 29 °C for 4 days. The guts of flies were dissected and labeled with anti-GFP (green), anti-Delta (white), and anti-γH2AvD (red) antibodies and DAPI (blue). Yellow arrow heads indicate Delta⁺ cell. Upper two panels is merged image. Lower four panels is gray scale image of upper images. Asterisk indicates Myo^-, Delta^-, and strong γH2AvD⁺ cell, shows dying cell.