Research Paper Volume 10, Issue 2 pp 197—211

Figure 8. Sirt1 inhibition depressed autophagy and caused an over loss of germ cells. (A) Level of SIRT1 protein in control, EX527 and Sirt1 RNAi treatment for 2 days ovaries. (B) Level of H4K16ac protein in control, EX527 and Sirt1 RNAi treatment for 2 days ovaries. (C) WB analysis of LC3II/LC3I in control, EX527 and Sirt1 RNAi treated ovaries (6 h). (D) WB analysis of BAX/BCL-2 in control, EX527 and Sirt1 RNAi treated ovaries for 3 days. (E) IF staining for MVH (red) of control, EX527 and Sirt1 RNAi treated mouse ovaries for 3 days and 5 days. (F) Percentage of germ cells in cysts and follicles in the three groups after 3 days treatment. (G) Average number of survived oocytes in the three groups after 3 days treatment. (H) Percentage of germ cells in cysts and follicles in the three groups after 5 days treatment. (I) Average number of survived oocytes in the three groups after 5 days treatment. The results are presented as mean ± SD. * P < 0.05; ** P < 0.01.