Figure 2. The influence of senescence on expression and processing of APP in human BMECs. Human BMECs were passaged 5 and 15 times. Cell lysates were subjected to Western blot. Protein levels of (A) APP, (B) ADAM10, (C) ADAM17, (D) ADAM9 and (F) BACE1 were measured, n=6-9. Cells were incubated in 1.5ml EGM2 for 24h. Conditioned media were collected for measuring sAPPα (E, n=13) and Aβ40 (G, n=15) via commercially available ELISA kits respectively. Data are presented as mean±SEM, *P<0.05, compared to P5.